Zoeken
Zoeken kan via de modus 'eenvoudig zoeken' (één veld) of uitgebreid via 'geavanceerd zoeken' (meerdere velden). Zo kan je bv. zoeken op een combinatie van een auteursnaam (auteur), een jaartal (jaar) en een documenttype.
Boekenmand
Nuttige resultaten kan je aanvinken en toevoegen aan een mandje. De inhoud hiervan kan je exporteren of afdrukken (naar bv. PDF).
RSS
Op de hoogte blijven van nieuw toegevoegde publicaties binnen uw interessegebied? Dit kan door een RSS-feed (?) te maken van jouw zoekopdracht.
nieuwe zoekopdracht
| one publication added to basket [257228] |
Community structure and PAH ring-hydroxylating dioxygenase genes of a marine pyrene-degrading microbial consortium
Gallego, S.; Vila, J.; Tauler, M.; Nieto, J.; Breugelmans, P.; Springael, D.; Grifoll, M. (2014). Community structure and PAH ring-hydroxylating dioxygenase genes of a marine pyrene-degrading microbial consortium. Biodegradation 25(4): 543-556. dx.doi.org/10.1007/s10532-013-9680-z
In: Biodegradation. Springer: Dordrecht. ISSN 0923-9820; e-ISSN 1572-9729
|  |
| Trefwoorden |
Gordonia (ex Tsukamura, 1971) Stackebrandt, Smida & Collins, 1989 [WoRMS]
Marien/Kust |
| Author keywords |
Biodegradation; Pyrene; PAHs; Marine microbial consortia; Gordonia;Dioxygenase; Community structure analysis |
| Auteurs | | Top |
- Gallego, S.
- Vila, J.
- Tauler, M.
- Nieto, J.
|
- Breugelmans, P.
- Springael, D.
- Grifoll, M.
|
|
| Abstract |
Marine microbial consortium UBF, enriched from a beach polluted by the Prestige oil spill and highly efficient in degrading this heavy fuel, was subcultured in pyrene minimal medium. The pyrene-degrading subpopulation (UBF-Py) mineralized 31 % of pyrene without accumulation of partially oxidized intermediates indicating the cooperation of different microbial components in substrate mineralization. The microbial community composition was characterized by culture dependent and PCR based methods (PCR-DGGE and clone libraries). Molecular analyses showed a highly stable community composed by Alphaproteobacteria (84 %, Breoghania, Thalassospira, Paracoccus, and Martelella) and Actinobacteria (16 %, Gordonia). The members of Thalasosspira and Gordonia were not recovered as pure cultures, but five additional strains, not detected in the molecular analysis, that classified within the genera Novosphingobium, Sphingopyxis, Aurantimonas (Alphaproteobacteria), Alcanivorax (Gammaproteobacteria) and Micrococcus (Actinobacteria), were isolated. None of the isolates degraded pyrene or other PAHs in pure culture. PCR amplification of Gram-positive and Gram-negative dioxygenase genes did not produce results with any of the cultured strains. However, sequences related to the NidA3 pyrene dioxygenase present in mycobacterial strains were detected in UBF-Py consortium, suggesting the representative of Gordonia as the key pyrene degrader, which is consistent with a preeminent role of actinobacteria in pyrene removal in coastal environments affected by marine oil spills. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
