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Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.

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one publication added to basket [71305]
Measurement of EROD activity: caution on spectral properties of standards used Radenac, G.; Coteur, G.; Danis, B.; Dubois, P.; Warnau, M. (2004). Measurement of EROD activity: caution on spectral properties of standards used. Mar. Biotechnol. 6(4): 307-311. dx.doi.org/10.1007/s10126-004-3014-4 In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228; e-ISSN 1436-2236, meer

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Beschikbaar in	Auteurs
VLIZ: Open Marine Archive 280392 [ download pdf ]

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Trefwoorden

Analysis > Mathematical analysis > Spectral analysis Biomarkers Fluorimeters Marien/Kust

Author keywords

EROD; ethoxyresorufin; resorufin; spectral characteristics; fluorimetry; biomarker

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Auteurs		Top
Radenac, G. Coteur, G. Danis, B., meer	Dubois, P., meer Warnau, M.

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Abstract

The activity of the enzyme 7-ethoxy-resorufin-O-deethylase (EROD) has been extensively used in biomonitoring studies for more than a decade. Although the analytical procedure is simple, it is often poorly characterized. In this study spectral properties of particular standard compounds used to measure EROD activity (ethoxyresorufin and resorufin, standards from Molecular Probes) were tested in order to optimize excitation and emission wavelengths to be used in the fluorimetric assay of EROD activity. The optimal excitation wavelength for the detection of resorufin was 560 nm. At this wavelength the excitation represents only 37% of its maximum level for ethoxyresorufin, while it represents 86% for resorufin. This allows discrimination between the fluorescence emitted by both standards, favoring the formed resorufin. Our results demonstrate that any analytical work using spectrofluorometry to measure EROD activity should be preceded by precise determination of the spectral characteristics of each set of standards used.

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