Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
| one publication added to basket [392992] |
The influence of ocean acidification and species shifts on algal exudate production: Case study Alexandrium minutum, Protoceratium reticulatum, Phaeodactylum tricornutum
Van de Weyer, N. (2024). The influence of ocean acidification and species shifts on algal exudate production: Case study Alexandrium minutum, Protoceratium reticulatum, Phaeodactylum tricornutum
. MSc Thesis. Ghent University, Faculty of Bioscience Engineering: Gent. 104 pp.
|
| Beschikbaar in | Auteur |
|
Documenttype: Doctoraat/Thesis/Eindwerk
|
| Trefwoorden |
Alexandrium minutum Halim, 1960 [WoRMS]; Phaeodactylum tricornutum Bohlin, 1897 [WoRMS]; Protoceratium reticulatum (Claparède & Lachmann) Bütschli, 1885 [WoRMS]
Marien/Kust |
| Abstract |
In this research, three algal species—the diatom Phaeodactylum tricornutum and the dinoflagellates Alexandrium minutum and Protoceratium reticulatum—were exposed to different levels of acidification in their growth medium. The objective was to investigate the impact of ocean acidification, a consequence of climate change, on their growth and exudate production. The study quantified 2 classes of exudates: Extracellular Polymeric Substances (EPS) through an extraction method followed by Bradford reagent colouring and Transparent Extracellular Particles (TEP), a planktonic subclass of EPS, by staining with Alcian Blue. Understanding how environmental changes affect EPS production is vital, as EPS plays a key role in the structure and functionality of microbial biofilms, aiding in surface adhesion and providing a protective buffer for organisms. The protein content in EPS is particularly important for these functions. Therefore, ATR spectra were also used to determine the protein-to-carbohydrate (P/C) ratio over time and compare it across different pH treatments (pH 8.10, pH 7.80, and pH 6.37). The impact of ocean acidification on TEP production is also important to understand since this will impact the vertical carbon flux in the marine water column. The findings illustrate that cell density measurements under varying pH conditions revealed species-specific responses: Phaeodactylum tricornutum showed increased growth at moderate acidification compared to severely acidified or normal conditions, while Alexandrium minutum and Protoceratium reticulatum displayed varied but insignificant growth rate changes. EPS production generally increased under acidified conditions, with species-specific trends (logarithmic, linear, exponential). TEP production initially rose at lower pH levels but later decreased, except for Protoceratium reticulatum. EPS quality, characterized by protein-to-carbohydrate ratio, also showed species-specific responses to pH changes. No clear conclusions could be drawn however, due to the big interspecies variability and a lot of hiatuses still present in the used methodology, making statistical analysis hard at times. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
