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| one publication added to basket [280992] |
The first green lineage cdc25 dual-specificity phosphatase
Khadaroo, B.; Robbens, S.; Ferraz, C.; Derelle, E.; Eychenie, S.; Cooke, R.; Peaucellier, G.; Delseny, M.; Demaille, J.; Van de Peer, Y.; Picard, A.; Moreau, H. (2004). The first green lineage cdc25 dual-specificity phosphatase. Cell Cycle 3(4): 513-518
In: Cell Cycle. TAYLOR & FRANCIS INC: Philadelphia. ISSN 1538-4101; e-ISSN 1551-4005, meer
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| Trefwoord |
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| Author keywords |
Cdc25; alga; plant; cell division; cell cycle; regulation; control;G(2)/M; transition; MPF |
| Auteurs | | Top |
- Khadaroo, B.
- Robbens, S.
- Ferraz, C.
- Derelle, E.
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- Eychenie, S.
- Cooke, R.
- Peaucellier, G.
- Delseny, M.
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- Demaille, J.
- Van de Peer, Y., meer
- Picard, A.
- Moreau, H.
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| Abstract |
The Cdc25 protein phosphatase is a key enzyme involved in the regulation of the G(2)/M transition in metazoans and yeast. However, no Cdc25 ortholog has so far been identified in plants, although functional studies have shown that an activating dephosphorylation of the CDK-cyclin complex regulates the G(2)/M transition. In this paper, the first green lineage Cdc25 ortholog is described in the unicellular alga Ostreococcus tauri. It encodes a protein which is able to rescue the yeast S. pombe cdc25-22 conditional mutant. Furthermore, microinjection of GST-tagged O. tauri Cdc25 specifically activates prophase-arrested starfish oocytes. In vitro histone H1 kinase assays and anti-phosphotyrosine Western Blotting confirmed the in vivo activating dephosphorylation of starfish CDK1-cyclinB by recombinant O. tauri Cdc25. We propose that there has been coevolution of the regulatory proteins involved in the control of M-phase entry in the metazoan, yeast and green lineages. |
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