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Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
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| one publication added to basket [247099] |
Haloperoxidase mediated quorum quenching by Nitzschia cf pellucida: study of the metabolization of N-Acyl Homoserine Lactones by a benthic diatom
Syrpas, M.; Ruysbergh, E.; Blommaert, L.; Vanelslander, B.; Sabbe, K.; Vyverman, W.; De Kimpe, N.; Mangelinckx, S. (2014). Haloperoxidase mediated quorum quenching by Nitzschia cf pellucida: study of the metabolization of N-Acyl Homoserine Lactones by a benthic diatom. Mar. Drugs 12(1): 352-367. dx.doi.org/10.3390/md12010352
In: Marine Drugs. Molecular Diversity Preservation International (MDPI): Basel. ISSN 1660-3397; e-ISSN 1660-3397, meer
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| Trefwoorden |
Nitzschia pellucida Grunow, 1880 [WoRMS]
Marien/Kust |
| Author keywords |
quorum sensing; haloperoxidase; degradation pathway; AHL; referencecompounds; diatom-bacteria interactions |
| Abstract |
Diatoms are known to produce a variety of halogenated compounds, which were recently shown to have a role in allelopathic interactions between competing species. The production of these compounds is linked to haloperoxidase activity. This research, has shown that this system may also be involved in diatom-bacteria interactions via the H2O2 dependent inactivation of a type of quorum sensing (QS) molecule, i.e., N-ß-ketoacylated homoserine lactones (AHLs), by a natural haloperoxidase system from the benthic diatom Nitzschia cf pellucida. The AHL degradation pathway towards corresponding halogenated derivatives was elucidated via HPLC-MS analysis and the synthesis of a broad series of novel halogenated AHL analogues as reference compounds. Furthermore, their biological activity as quorum sensing modulators was directly compared and evaluated against a series of naturally occurring ß-keto-AHLs. It has been demonstrated that the loss of the QS activity results from the final cleavage of the halogenated N-acyl chain of the signal molecules. |
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