Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
| one publication added to basket [239259] |
Dual BrdU-PCNA immunodetection of proliferative cells in dental and orofacial tissues of teleosts
Vandenplas, S.; Willems, M.; Huysseune, A. (2012). Dual BrdU-PCNA immunodetection of proliferative cells in dental and orofacial tissues of teleosts. J. Appl. Ichthyol. 28(3): 336-340. dx.doi.org/10.1111/j.1439-0426.2012.02003.x
In: Journal of Applied Ichthyology = Zeitschrift für angewandte Ichthyologie. Blackwell: Berlin. ISSN 0175-8659; e-ISSN 1439-0426, meer
|  |
| Abstract |
We have developed a protocol for simultaneous detection of BrdU and PCNA on paraffin sections of teleost tissues and have tested it successfully on dental and orofacial tissues of Atlantic salmon (Salmo salar) and zebrafish (Danio rerio). By combining decalcification of the tissue, appropriate antigen retrieval steps, and high quality tissue sections, this protocol allows to study the instantaneous pattern of proliferating cells, as well as cell tracing in large specimens. The technique is therefore highly suitable to study skeletal and dental tissues, tissues that are traditionally not easily accessible to molecular and cellular techniques. Dual BrdU-PCNA immunostaining may serve as a useful tool in experimental studies on fish developmental or regenerative processes such as tissue turnover in orofacial epithelia, continuous tooth replacement, and in the identification of putative stem cells for which the cycling state of BrdU label retaining cells needs to be determined. |
IMIS is ontwikkeld en wordt gehost door het VLIZ.
