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Growth kinetics of Listeria isolated from Salmon and Salmon processing environment: single strains versus cocktails
Skåra, T.; Cappuyns, A.M.; Van Derlinden, E.; Rosnes, J.T.; Valdramidis, V.P.; Van Impe, J.F.M. (2012). Growth kinetics of Listeria isolated from Salmon and Salmon processing environment: single strains versus cocktails. J. Food Prot. 75(7): 1227-1235. dx.doi.org/10.4315/0362-028X.JFP-11-536
In: Journal of Food Protection. IAFP: Ames, Iowa,. ISSN 0362-028X; e-ISSN 1944-9097, meer
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| Auteurs | | Top |
- Skåra, T., meer
- Cappuyns, A.M., meer
- Van Derlinden, E., meer
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- Rosnes, J.T.
- Valdramidis, V.P.
- Van Impe, J.F.M., meer
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| Abstract |
The growth dynamics of Listeria monocytogenes strains isolated from salmon or a salmon processing environment and two reference Listeria innocua strains were investigated at refrigerated and close-to-optimal growth temperatures. Estimates for the growth rates and the lag-phase duration at 4, 8, 12, and 30°C were obtained for optical density measurements by using different growth parameter estimation methods, i.e., the serial dilution (SD) method and the relative rate to detection (RRD) method. Both single L. innocua and L. monocytogenes strains and mixtures of L. monocytogenes strains (cocktails) were studied. Both methods show an increase in maximum growth rate (µmax) of Listeria with increasing temperatures. Generally, single-strain growth rate estimates were quite similar for both species, althoughL. monocytogenes showed slightly higher µmax estimates at 4°C. The SD method gave the highest estimates for the growth rate, i.e., the estimates from the RRD method were 10 to 20% lower. This should lead to caution when using the latter method for Listeria, particularly at lower temperatures. Overall, the SD method is preferred as this method yields µmax estimates close to the biological value and provides estimates for the duration of lag time (?). For discrimination between different strains, ? appeared to be a more suitable parameter than µmax. This effect was most prominent for L. innocua. Significant differences were observed between µmax and/or ? of L. monocytogenes cocktails and single strains at all temperatures investigated. At 4°C, the average growth rate of cocktails was higher than that of single strains. At 8 and 30°C, this trend was reversed. The average ? of single strains were more than twice as long as those of cocktails at 4°C. At 8 and 30°C, the ? of cocktails were significantly slower than those of single strains, but the variation was considerably less and the differences were less pronounced. |
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