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Selection of marine bacterial consortia efficient at degrading chitin leads to the discovery of new potential chitin degraders
Meunier, L.; Costa, R.; Keller-Costa, T.; Cannella, D.; Dechamps, E.; George, I.F. (2024). Selection of marine bacterial consortia efficient at degrading chitin leads to the discovery of new potential chitin degraders. Microbiology Spectrum 12(11): 1-20. https://dx.doi.org/10.1128/spectrum.00886-24
In: Microbiology Spectrum. ASM Press: Washington. e-ISSN 2165-0497, meer
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| Author keywords |
microbial communities, enrichment cultures, chitinase, marine sponge, size exclusion chromatography |
| Auteurs | | Top |
- Meunier, L., meer
- Costa, R.
- Keller-Costa, T.
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- Cannella, D.
- Dechamps, E., meer
- George, I.F., meer
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| Abstract |
Chitin degradation is a keystone process in the oceans, mediated by marine microorganisms with the help of several enzymes, mostly chitinases. Sediment, seawater, and filter-feeding marine invertebrates, such as sponges, are known to harbor chitin-degrading bacteria and are presumably hotspots for chitin turnover. Here, we employed an artificial selection process involving enrichment cultures derived from microbial communities associated with the marine sponge Hymeniacidon perlevis, its surrounding seawater and sediment, to select bacterial consortia capable of degrading raw chitin. Throughout the artificial selection process, chitin degradation rates and the taxonomic composition of the four successive enrichment cultures were followed. To the best of our knowledge, chitin degradation was characterized for the first time using size exclusion chromatography, which revealed significant shifts in the numbered average chitin molecular weight, strongly suggesting the involvement of endo-chitinases in the breakdown of the chitin polymer during the enrichment process. Concomitantly with chitin degradation, the enrichment cultures exhibited a decrease in alpha diversity compared with the environmental samples. Notably, some of the dominant taxa in the enriched communities, such as Motilimonas, Arcobacter, and Halarcobacter, were previously unknown to be involved in chitin degradation. In particular, the analysis of published genomes of these genera suggests a pivotal role of Motilimonas in the hydrolytic cleavage of chitin. This study provides context to the microbiome of the marine sponge Hymeniacidon perlevis in light of its environmental surroundings and opens new ground to the future discovery and characterization of novel enzymes of marine origin involved in chitin degradation processes. |
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