Over het archief
Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.
Dit archief wordt uitgebouwd en beheerd volgens de principes van de Open Access Movement, en het daaruit ontstane Open Archives Initiative.
Basisinformatie over ‘Open Access to scholarly information'.
| [ meld een fout in dit record ] | mandje (0): toevoegen | toon |
 |
| Archaeal DNA-import apparatus is homologous to bacterial conjugation machinery Beltran, L.C.; Cvirkaite-Krupovic, V.; Miller, J.; Wang, F.; Kreutzberger, M.A.B.; Patkowski, J.B.; Costa, T.R.D.; Schouten, S.; Levental, I.; Conticello, V.P.; Egelman, E.H.; Krupovica, M. (2023). Archaeal DNA-import apparatus is homologous to bacterial conjugation machinery. Nature Comm. 14: 666. https://dx.doi.org/10.1038/s41467-023-36349-8
In: Nature Communications. Nature Publishing Group: London. ISSN 2041-1723; e-ISSN 2041-1723, meer
|   |
| Beschikbaar in | Auteurs |
| Auteurs | Top | |
|
|
|
| Abstract |
Conjugation is a major mechanism of horizontal gene transfer promoting the spread of antibiotic resistance among human pathogens. It involves establishing a junction between a donor and a recipient cell via an extracellular appendage known as the mating pilus. In bacteria, the conjugation machinery is encoded by plasmids or transposons and typically mediates the transfer of cognate mobile genetic elements. Much less is known about conjugation in archaea. Here, we determine atomic structures by cryo-electron microscopy of three conjugative pili, two fromhyperthermophilic archaea (Aeropyrum pernix and Pyrobaculum calidifontis) and one encoded by the Ti plasmid of the bacterium Agrobacterium tumefaciens, and show that the archaeal pili are homologous to bacterial mating pili. However, the archaeal conjugation machinery, known as Ced, has been ‘domesticated’, that is, the genes for the conjugation machinery are encoded on the chromosome rather than on mobile genetic elements, and mediates the transfer of cellular DNA. |
| Top | Auteurs |
