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Non-lethal heat shock protects gnotobiotic Artemia franciscana larvae against virulent Vibrios Yik Sung, Y.; Van Damme, E.J.M.; Sorgeloos, P.; Bossier, P. (2007). Non-lethal heat shock protects gnotobiotic Artemia franciscana larvae against virulent Vibrios. Fish Shellfish Immunol. 22(4): 318-326. dx.doi.org/10.1016/j.fsi.2006.05.008 In: Fish & Shellfish Immunology. Academic Press: London; New York. ISSN 1050-4648; e-ISSN 1095-9947, meer

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Beschikbaar in	Auteurs
VLIZ: Open access 262522 [ download pdf ]

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Trefwoorden

Biological phenomena > Biosynthesis Biology > Microbiology Biology > Physiology Developmental stages > Larvae Environmental effects > Temperature effects > Heat shock Heat shock proteins Immune response Pathogens Peptides > Proteins > Heat shock proteins Survival Virulence Artemia franciscana Kellog, 1906 [WoRMS]; Vibrio campbellii (Baumann, Baumann & Mandel, 1971) Baumann, Baumann, Bang & Woolkalis, 1981 [WoRMS]; Vibrio proteolyticus (Merkel et al., 1964) Baumann et al., 1982 [WoRMS] Marien/Kust

Author keywords

non-lethal heat-shock; heat shock proteins; Artemia franciscana; immune response; challenge test; Vibrio campbellii; Vibrio proteolyticus

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Auteurs		Top
Yik Sung, Y., meer Van Damme, E.J.M., meer Sorgeloos, P., meer Bossier, P., meer

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Abstract

Brine shrimp Artemia were exposed under gnotobiotic conditions to a non-lethal heat shock (NLHS) from 28 to 32, 37 and 40°C. Different recovery periods (2, 6, 12 and 24 h) and different heat-exposure times (15, 30, 45 and 60 min) were tested. After these NLHS, Artemia was subsequently challenged with Vibrio. Challenge tests were performed in stressed and unstressed nauplii at concentrations of 107 cells ml-1 of pathogenic bacteria, Vibrio campbellii and Vibrio proteolyticus. A NLHS with an optimal treatment of 37°C for 30 min and a subsequent 6 h recovery period resulted in a cross-protection against pathogenic Vibrio. A 100% increase in the larval survival (P < 0.05) was observed. We have also demonstrated by Western blot that a NLHS increases the expression of HSP-70 in heat-shocked (HS) treated animals. This report is the first to reveal a cross protection of a NLHS against deleterious bacterial challenges in living crustaceans. The putative role of heat shock proteins (HSPs) in this process is discussed.

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