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Het OWA, het open archief van het Waterbouwkundig Laboratorium heeft tot doel alle vrij toegankelijke onderzoeksresultaten van dit instituut in digitale vorm aan te bieden. Op die manier wil het de zichtbaarheid, verspreiding en gebruik van deze onderzoeksresultaten, alsook de wetenschappelijke communicatie maximaal bevorderen.

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Pluripotency and chimera competence of an embryonic stem cell line from the sea perch (Lateolabrax japonicus) Chen, S.-L.; Sha, Z.X.; Ye, H.-Q.; Liu, Y.; Tian, Y.-S.; Hong, Y.; Tang, Q.-S. (2007). Pluripotency and chimera competence of an embryonic stem cell line from the sea perch (Lateolabrax japonicus). Mar. Biotechnol. 9(1): 82-91. http://dx.doi.org/10.1007/s10126-006-6050-1 In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228; e-ISSN 1436-2236, meer

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Beschikbaar in	Auteurs
VLIZ: Open access 123194 [ download pdf ]

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Trefwoorden

Cells > Embryonic stem cells Lateolabrax japonicus (Cuvier, 1828) [WoRMS] Marien/Kust

Author keywords

cell transplantation; chimera; embryonic stem cells; pluripotency; seaperch; zebrafish

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Auteurs		Top
Chen, S.-L. Sha, Z.X. Ye, H.-Q. Liu, Y., meer	Tian, Y.-S. Hong, Y. Tang, Q.-S.

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Abstract

A stable GFP-expressing (GFP+LJES1) cell strain was developed from the LJES1 cells obtained from sea perch (Lateolabrax japonicus,) embryos. GFP+LJES1 cells were induced in vitro by RA to differentiate into a variety of cell types and also had the ability to form embryoid body-like structures in suspension culture. To determine the differentiation potential of LJES1 cells in vivo, GFP+LJES1 cells were transplanted into sea perch and zebrafish embryos at mid-blastula stage. Twenty out of 478 transplanted sea perch embryos contained GFP-expressing LJES1 cells 24 h after microinjection. Fifteen chimera embryos developed into fry. In these chimeras, the GFP+LJES1 cells contributed to a variety of tissues including the head and trunk. In zebrafish, 221 embryos were microinjected with GFP+LJES1 cells and 22 chimera embryos and fries expressing GFP were obtained. Donor GFP+LJES1 cells contributed to various tissues in head and trunk of zebrafish embryos and hatched fry.

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