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Molecular responses during cadmium-induced stress in Daphnia magna: integration of differential gene expression with higher-level effects
Soetaert, A.; Vandenbrouck, T.; Van der Ven, K.; Maras, M.; van Remortel, P.; Blust, R.; De Coen, W.M. (2007). Molecular responses during cadmium-induced stress in Daphnia magna: integration of differential gene expression with higher-level effects. Aquat. Toxicol. 83(3): 212-222. dx.doi.org/10.1016/j.aquatox.2007.04.010
In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X; e-ISSN 1879-1514
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| Trefwoorden |
Chemical elements > Metals > Heavy metals > Cadmium
Disciplines > Biology > Molecular biology > Genetics > Genomics > Functional genomics > Toxicology > Toxicogenomics
Energy budget
Daphnia magna Straus, 1820 [WoRMS]
Zoet water |
| Author keywords |
toxicogenomics; microarray; cadmium; energy budget; Daphnia magna; multiple levels of biological organization |
| Auteurs | | Top |
- Soetaert, A.
- Vandenbrouck, T.
- Van der Ven, K.
- Maras, M.
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- van Remortel, P.
- Blust, R.
- De Coen, W.M.
|
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| Abstract |
DNA microarrays offer great potential in revealing insight into mechanistic toxicity of contaminants. The aim of the present study was (i) to gain insight in concentration- and time-dependent cadmium-induced molecular responses by using a customized Daphnia magna microarray, and (ii) to compare the gene expression profiles with effects at higher levels of biological organization (e.g. total energy budget and growth). Daphnids were exposed to three cadmium concentrations (nominal value of 10, 50, 100 µg/l) for two time intervals (48 and 96 h). In general, dynamic expression patterns were obtained with a clear increase of gene expression changes at higher concentrations and longer exposure duration. Microarray analysis revealed cadmium affected molecular pathways associated with processes such as digestion, oxygen transport, cuticula metabolism and embryo development. These effects were compared with higher-level effects (energy budgets and growth). For instance, next to reduced energy budgets due to a decline in lipid, carbohydrate and protein content, we found an up-regulated expression of genes related to digestive processes (e.g. a-esterase, cellulase, a-amylase). Furthermore, cadmium affected the expression of genes coding for proteins involved in molecular pathways associated with immune response, stress response, cell adhesion, visual perception and signal transduction in the present study. |
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